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KMID : 0357319870220010001
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Journal of the Korean Society for Microbiology
1987 Volume.22 No. 1 p.1 ~ p.8
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Study on the Pathogenesis of Hantaan Virus with Monoclonal Antibodies
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Kim Gum-Ryong
Kim Tai-Gyu
Rhyu Mun-Gan
Lim Byung-Uk
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Abstract
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Hantaan virus(HV) 76-118 strain was inoculated into suckling ICR mice by intra-nasal route with an inoculum of 10LD50. Mortality was 65% at the 3rd week after inoculation, but declined to 35% at the 4th week. Infectivity was determined by the measuring immuno-fluorescent antibody in sera. The peak of infectivity was 80% at the 4’th week after inoculation. Viremia was reached peak level of 1.7×104PFU/ml by day 10. Immunofluorescent antibody and neutralizing antibody appeared by 2 weeks and 15-17 days respectively, but achieved similar titer by 35 days. By using a monoclonal antibody to HV 76-118, viral antigens were initially detected in inguinal and axillary lymph node by 2 days. Viral antigens in bone marrow and lung were delayed much more than in those of lymph node. These were similar with those of intra-peritoneal and intra-muscular route. Immune complex against IgG, IgM and C3 appeared by 16 days, 14 days, and 18 days respectively. The pattern of immunofluorescence in the basement membrane of glomeruli was diffuse membranous. Spotted pattern was also observed in the tissue stained with anti-mouse C3 antibody. By 20 days, control tissue was also shown immune complex in the glomeruli.
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KEYWORD
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Hantaan virus, pathogenesis, suckling mice, monoclonal antibody, lymph node, complex
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